Hermaphrodite hook up site
The active agent in the gland mucus fulfills the requirements for the substance to be classified as an allohormone; it enters the circulatory system of the conspecific via inoculation by the dart and stimulates physiological and behavioral changes (20).
In this study, we analyzed the contents of the mucous glands of ) were purchased from commercial culturists (Glasshouse Gourmet Snails), transported, and stored at University of the Sunshine Coast helicid facilities during the months of April and May in 20.
were obtained from Warooka, South Australia, and the grains belt of South Yorke Peninsula, South Australia, and transported to the University of the Sunshine Coast helicid facilities.
All snails were housed in moist nylon mesh-covered pens on a 12-h light/dark photoperiod and fed alternating days with slices of cucumber or carrot until used for bioassay.
In this study, we used the common garden snail to identify the allohormone that is responsible for this physiological change in the female system of this simultaneous hermaphrodite.
The love dart allohormone (LDA) was isolated from extracts derived from mucous glands that coat the dart before it is stabbed through the partner's body wall.
Acting as a hypodermic needle, the dart transfers an allohormone that increases paternity success.
Its precise physiological mechanism of action within the recipient snail is to close off the entrance to the sperm digestion organ via a contraction of the copulatory canal, thereby delaying the digestion of most donated sperm.
This was later disproved, because the amount of calcium in one dart is roughly equal to that of one egg, and thus it would not contribute significantly to an average clutch of 59 eggs (for ) (18).
The physiological mechanism of action seems to be through reconfiguration of the spermatophore-receiving organ copulatory canal via peristaltic movements, which in turn increases the number of escaping spermatozoa (13).
Because the love darts alone do not increase paternity success, the mucus is implicated as a carrier of the active substance(s) (10).
In all assays, statistical analysis was performed using Pearson's chi-square test or Fisher's exact test as appropriate.
In all assays, the and placed into separate glass convex dishes containing 1 ml of chilled Milli Q water treated with 0.1% TFA.
However, this is still followed by the injection of a calcareous love dart and copulation.